Desaturation of stearate by sheep tissue microsomal preparations
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منابع مشابه
Dietary effects on the formation of dolichyl monophosphate mannose by microsomal preparations of rat adiopose tissue.
Microsomal preparations from rat adipose tissue catalyse the transfer of [(14)C]mannose from GDP-[(14)C]mannose to an endogenous acceptor forming a [(14)C]mannosyl lipid. The mannosyl lipid co-chromatographs with hen oviduct dolichyl monophosphate beta-mannose on three solvent systems. It is stable to mild alkaline hydrolysis, but strong alkaline treatment yields a compound that co-migrates wit...
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A cell-free particulate preparation from oyster viscera incorporated [r4C]palmitate and [r4C]serine into the long chain base sphingadienine. Both double bonds of the product were shown to have the frans configuration. frans2-Hexadecenoic and frans-6-hexadecenoic acids were not effective in decreasing the incorporation of the radioactive label from palmitate, nor was label from palmitate incorpo...
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Neoplastic mast cells of mice (including long-established and newly derived lines) were grown in large-volume suspension cultures to provide enough cells for preparation of microsomal fractions. Microsomal preparations from P815Y and P815S cells synthesized (14)C-labelled glycosaminoglycan when incubated with UDP-[(14)C]glucuronic acid and UDP-N-acetylgalactosamine. No significant amount of (14...
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by rat liver preparations is shown in Scheme 1, and is similar to that proposed by Jerina et al. (1968) for the metabolism of naphthalene. In both cases the action of the epoxide hydratase leads to the production of the trans-diol only. The occurrence of ris-indane-f,2-diol in addition to the trans-diol as a metabolite of indene in the rat (Brooks &Young, 1956) could be explained by the metabol...
متن کاملRemoval of plasma-membrane fragments from rat liver microsomal preparations by agarose-bound concanavalin A
The membrane fraction of a rat liver post-mitochondria1 supernatant is largely derived from endoplasmic reticulum, but also contains plasma-membrane fragments and Golgi membranes (see, e.g., Depierre & Dallner, 1975). The subfractionation of microsomal preparations normally depends on the use of high centrifugal fields, and hence large hydrostatic pressures. We have attempted to avoid these har...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1972
ISSN: 0306-3283
DOI: 10.1042/bj1290015pb